pleasei need help with these i’m doing my quiz
1) The best fit curve line to an absorbance vs. concentration plot for standard solutions of a dye has a slope of 0.2567 mL/mg and intercept of 0.0500. A sample solution prepared of the same dye has an absorbance of 0.675 at the wavelength used to establish the Beer’s law plot. What is the concentration of dye in the prepared solution?
a. 0.675 mg/mLb. 2.82 mg/mLc. 0.223 mg/mLd. 0.173 mg/mLe. 2.43 mg/mL
2) Calculate the concentration of a solution prepared by transferring 25.00 mL of a 10.6 mg/mL dye solution to a 50.00 mL volumetric flask and adding water to the calibration mark. Select one:a. 10.6 mg/mLb. 5.30 mg/mLc. 0.0472 mg/mLd. 0.00848 mg/mLe. 21.2 mg/mL
Which of the following will interfere with an absorbance measurement?1. fingerprints on the outside of the cuvette2. scratches on the cuvette3. aclear colorless solution4. air bubbles5. thecuvette filled to the top6. liquid on the outside of the cuvette
Put the following steps in the correct sequence to calibrate a Spectronic-20.1. Set the instrument to the desired wavelength.2. Adjust the light control or transmittance/absorbance knob to 100% transmittance3. Place the reference cuvette in the cuvette holder.4. Remove reference cuvette from the cuvette holder.5. Set the % transmittance to zero using the amplifier or zero control knob.