In Figure 2A the authors are using a transcriptional pulse chase to trap mRNA degradation intermediates.

In Figure 2A the authors are using a transcriptional pulse chase to trap mRNA degradation intermediates. They use a galactose inducible promoter to initiate new mRNA synthesis in the presence of radiolabeled nucleotides (the pulse). After 10 minutes (when the mRNA synthesis is complete) they remove the radiolabeled nucleotides and monitor degradation products of the newly synthesized radiolabeled mRNA (the chase). The polyG sequence is inserted at the the BglII site at position 28 (B28pGM). The pC marks the poly C sequence in the probe. There are 2 polyC sequences one at position 28 which can hybridize with the poly G and another at an internal location which does not have a complementary polyG sequence to hybridize to.

  • Best Nursing Writers
  • Best Nursing Writing Company
  • Buy a Nursing Essay
  • Capstone Project Writing Services
  • CHEAP NURSING ASSIGNMENTS
  • Cheap Thesis Writing Nurses
  • Community Health
  • Course Work
  • Document Formatting Services
  • Family Health Nursing
  • Fundamentals of Nursing
  • Geriatrics
  • GRADUATES CAPSTONE PROJECT
  • Health & Human Ecology
  • Human Bio-Sciences
  • Human Physiology
  • Industry Warning and Guidance!
  • Legit Nursing Writing Company
  • Medical Writing Services
  • Mental Health & Psychiatry
  • Nursing Assignment Help
  • Nursing Bibliography
  • Nursing Case Study
  • Nursing Coursework Help
  • Nursing Dissertation Writing Services
  • Nursing Essay/Personal Statement
  • Nursing Essays
  • Nursing Freelance Writers
  • Nursing Practitioners
  • Nursing Report Writing
  • Nursing Surgery
  • Nursing Term Paper Writing
  • Nursing Thesis Writing

©  2020 Nursingpaperspros.com