In their landmark 1973 study, Block et al. performed an analysis of the development of 5-flurocytosine resistance in the pathogen Cryptococcus neoformans.
a) How did they determine if resistance is induced through interaction between the pathogen and the anti-fungal agent?
b) How could you have used this approach to determine whether exposure to creates lys mutants?
c) What result should you anticipate, and why?