The method of Northern blotting is used to determine the amount and size of a particular RNA transcribed in a given cell type. Alternative splicing (discussed in Chapter 16) produces mRNAs from the same gene that have different lengths. A Northern blot is shown here using a DNA probe that is complementary to the mRNA encoded by a particular gene. The mRNA in lanes 1 through 4 was isolated from different cell types, and equal amounts of total cellular mRNA were added to each lane.
Lane 1: mRNA isolated from nerve cells
Lane 2: mRNA isolated from kidney cells
Lane 3: mRNA isolated from spleen cells
Lane 4: mRNA isolated from muscle cells
The method of Northern blotting is used to determine the
Explain these results.