The technique of Western blotting can be used to detect

The technique of Western blotting can be used to detect proteins that are translated from a particular mRNA. This method is described in Chapter 20 and also in experimental question E5.
Let’s suppose a researcher was interested in the effects of mutations on the expression of a protein-encoding gene that encodes a protein we will call protein X. This protein is expressed in skin cells and contains 572 amino acids. Its molecular mass is approximately 68,600 Da, or 68.6 kDa. Make a drawing that shows the expected results of a Western blot using proteins isolated from the skin cells obtained from the following individuals:
Lane 1: A normal individual
Lane 2: An individual who is homozygous for a deletion, which removes the promoter for this gene
Lane 3: An individual who is heterozygous in which one gene is normal and the other gene has a mutation that introduces an early stop codon at codon 421
Lane 4: An individual who is homozygous for a mutation that introduces an early stop codon at codon 421
Lane 5: An individual who is homozygous for a mutation that changes codon 198 from a valine codon into a leucine codon

  • Best Nursing Writers
  • Best Nursing Writing Company
  • Buy a Nursing Essay
  • Capstone Project Writing Services
  • CHEAP NURSING ASSIGNMENTS
  • Cheap Thesis Writing Nurses
  • Community Health
  • Course Work
  • Document Formatting Services
  • Family Health Nursing
  • Fundamentals of Nursing
  • Geriatrics
  • GRADUATES CAPSTONE PROJECT
  • Health & Human Ecology
  • Human Bio-Sciences
  • Human Physiology
  • Industry Warning and Guidance!
  • Legit Nursing Writing Company
  • Medical Writing Services
  • Mental Health & Psychiatry
  • Nursing Assignment Help
  • Nursing Bibliography
  • Nursing Case Study
  • Nursing Coursework Help
  • Nursing Dissertation Writing Services
  • Nursing Essay/Personal Statement
  • Nursing Essays
  • Nursing Freelance Writers
  • Nursing Practitioners
  • Nursing Report Writing
  • Nursing Surgery
  • Nursing Term Paper Writing
  • Nursing Thesis Writing

©  2020 Nursingpaperspros.com